Objective To study the mechanism of the toxicity of celangulin against Aedes albopictus larvae and its influence on various aspects, such as the growth and development of mosquito larvae. Methods Conventional biological approaches were employed for determination of the toxicity and the impact on the larva growth and development: the changes in the gastrointestinal morphology were identified by HE staining; the antifeedant activity was measured through the colorimetric determination of chromium oxide. Results With increasing concentrations, celangulin had strengthening toxicity against the fourth instar larvae of Ae. albopictus, with the LC₅₀ of 22.66 (18.38-27.95) mg/L and LC₉₅ of 84.51 (60.87-142.27) mg/L. Celangulin could reduce the pupation rate and extend the average pupation time and the average eclosion time of Ae. albopictus larvae, though it had no significant effect on the eclose rate. As the concentration of celangulin increased, its antifeedant activity became stronger, leading to gastrointestinal morphological changes: brush border loss and midgut degeneration and disintergration. Conclusion Celangulin was able to kill Ae. albopictus larvae through gastrointestinal toxicity, antifeedant activity and contact toxicity, and inhibit their growth and development.

Objective To histochemically locate the lipid peroxidation induced by photosensitized α-terthienyl(α-T) in Aedes albopictus larvae. Methods Cold-schiff was employed to observe the Ae. albopictus larvae exposed to photosensitized α-T under optical microscope in comparison to the control group to evaluate the histochemical localization of lipid peroxidation. Results Tissues in the control group remaining unstained, the midgut epithelium, the peritrophic membrane, the brush border and the lumen of malpighian tubules were stained purple in the experimental group. Conclusion Photosensitized α-T could induce lipid peroxidation in tissues of Ae. albopictus larvae, involving primarily the midgut and malpighian tubules.

【Abstract】 Objective To investigate the culture of salivary gland cells from adult Aedes albopictus, and try to establish its primary culture and passage culture. Methods Salivary glands dissected under sterile condition were kept in Schneider medium containing 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml phytomycin, and cultured at 26 ℃ in the incubator. Results After incubation for three days, single cells could be observed around salivary gland, and the quantity of single cell increased after six days. It showed that the culture of salivary gland cells from Ae.albopictus adult could survive for a period in vitro. Conclusion The culture of salivary gland cells from Ae.albopictus adult could survive in vitro.